단백질의 거동

모르는 것 및 단어:

corroborate: 확증하다.

ion-exchange chromatography

https://www.youtube.com/watch?v=A8lTfhWdAwE

surface diffusion

https://doi.org/10.1016/j.micromeso.2011.01.015

 

Scientists have struggled to directly measure the movement of proteins, known as protein diffusion, in materials that include both solid and liquid components. They have also disagreed on how movement at the surface of the material contributes to protein movement when using ion-exchange chromatography, a laboratory and manufacturing method for separating materials based on their charge. Proteins can creep into the pores of resin beads used to perform ion-exchange chromatography and bind on the walls, based on charge.

has shown that surface diffusion in protein transport into ion-exchange beads depends on adsorption affinity—a measure of attraction between the two materials

The team used chromatography, mechanistic modeling, confocal microscopy and small-angle neutron scattering.

By understanding and exploiting protein surface diffusion in ion-exchange chromatography, researchers can build upon this work and develop methods to reduce waste during the expensive drug manufacturing process.

Protein diffusion on surfaces is an important phenomenon inside the body, too. Movement and fibrillation of amyloid-ß in the brain has been associated with neurogenerative diseases, for example, and protein surface diffusion can affect the performance of biosensors.

 

 

 

reference

Ohnmar Khanal et al. Estimating and leveraging protein diffusion on ion-exchange resin surfaces, Proceedings of the National Academy of Sciences (2020). DOI: 10.1073/pnas.1921499117